Immunogold Staining of Epoxy Resin Sections for Transmission Electron Microscopy (TEM)
نویسندگان
چکیده
منابع مشابه
Immunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM).
INTRODUCTIONA pre-embedding method of immunochemical staining is used if antigens are damaged by resin embedding, or if the best preservation of membranes is required. Applying immunogold reagents to sections of lightly fixed tissue, free of embedding medium, can be a very sensitive method of immunochemical staining. Cells or tissues are fixed as strongly as possible and then treated with a cry...
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Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable ele...
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It has been the experience of this laboratory that the lead hydroxide stain of Watson (1) gives excellent results in increasing contrast in thin sections of tissue, but that the stain is inconvenient to use because on contact with air it forms an insoluble precipitate which contaminates both the stored solution and the sections. 1 Contamination introduced in transferring the solution from the s...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2008
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5015